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p53 antibody (AA 20-25)

TP53 Reactivity: Human, Cow, Horse, Sheep, Cat WB, IHC (p), IP, IHC (fro), EIA Host: Mouse Monoclonal DO-1 unconjugated
Catalog No. ABIN120054
  • Target See all p53 (TP53) Antibodies
    p53 (TP53) (Tumor Protein P53 (TP53))
    Binding Specificity
    • 51
    • 41
    • 40
    • 39
    • 32
    • 30
    • 28
    • 25
    • 24
    • 24
    • 23
    • 20
    • 20
    • 20
    • 18
    • 17
    • 16
    • 16
    • 14
    • 13
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    AA 20-25
    Reactivity
    • 889
    • 270
    • 220
    • 110
    • 44
    • 34
    • 31
    • 18
    • 10
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Cow, Horse, Sheep, Cat
    Host
    • 678
    • 227
    • 6
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 612
    • 302
    • 1
    Monoclonal
    Conjugate
    • 510
    • 49
    • 39
    • 26
    • 25
    • 24
    • 24
    • 24
    • 22
    • 22
    • 22
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This p53 antibody is un-conjugated
    Application
    • 719
    • 336
    • 254
    • 216
    • 208
    • 149
    • 124
    • 122
    • 119
    • 61
    • 61
    • 32
    • 20
    • 17
    • 13
    • 12
    • 11
    • 10
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody recognizes the p53 tumour suppressor protein, binding to both wild type and mutant forms. Clone DO-1 binds to both wild type and mutant forms of the p53 protein found in various malignancies (Kern et al. 1992). p53 is important in multicellular organisms, where it regulates cell cycle progression to allow DNA repair or apoptosis in the case of irreparably damaged cells (Haupt et al. 2003) and thus functions as a tumor suppressor that is involved in preventing cancer. Mutations in the p53 gene are found in about half the cases of human cancer (Joerger and Fersht 2007). DO-1 recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, common to isoforms 1-3 of p53.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Horse, Bovine, Cat and Sheep.
    Purification
    Affinity Chromatography on Protein G.
    Immunogen
    Recombinant human p53. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line.
    Clone
    DO-1
    Isotype
    IgG2a
    Top Product
    Discover our top product TP53 Primary Antibody
  • Application Notes
    Flow Cyometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections (1/25): Heat induced epitope retrieval with 0.01 M citrate buffer, pH 6.0 is recommended. Positive Control Tissue: Colon or breast carcinoma. ELISA. Western Blotting (1/1000). Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    p53 (TP53) (Tumor Protein P53 (TP53))
    Alternative Name
    p53 (TP53) (TP53 Products)
    Synonyms
    BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
    Background
    Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
    Gene ID
    7157
    NCBI Accession
    NP_000537
    UniProt
    P04637
    Pathways
    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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