alpha Fetoprotein antibody

Catalog No. ABIN120683

The Mouse Monoclonal anti-alpha Fetoprotein antibody is suitable to detect alpha Fetoprotein in samples from Human. It has been validated for IHC (p), RIA and EIA.

Quick Overview for alpha Fetoprotein antibody (ABIN120683)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Fetoprotein antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Enzyme Immunoassay (EIA)

Clone: AFP-11

Product Details anti-alpha Fetoprotein Antibody

Specificity: The antibody detects Alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa.

Purification: Precipitation methods

Purity: > 95 % by SDS-PAGE

Immunogen: Purified alpha-Fetoprotein

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections). Positive tissue: hepatocellular carcinoma. ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-Fetoprotein in combination with antibody clone AFP-01 (cat. no. ABIN120681/PX). RIA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Background: AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP

Gene ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process