HTR4
Reactivity: Human, Rat, Mouse
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry with Formalin Fixed Paraffin Embedded Tissues: 6 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
The 5-HT4 receptor, a member of the Serotonin Receptor subfamily, has been shown to affect the sensory and motor functions of the gut. Some of the most promising emerging therapies in Irritable Bowel Syndrome revolve around targeted pharmacotherapeutic modulation of serotonin receptors (ie, 5-HT3 and 5-HT4 subtypes). At least 9 isoforms (5-HT4A-5-HT4G, and 5-HT4n) have been described, which differ in the length and composition of their intracellular C termini after a common splicing site (L358). A ninth isoform (5-HT4H) results in the insertion of 14 amino acids into the second intracellular loop. 5-HT4 receptor expression has been documented primarily in the brain, and in colon, heart, and vessel. An EST has been isolated from a human cancerous nerve library. Ligand: Serotonin.Synonyms: 5-HT-4, 5-HT4, 5-hydroxytryptamine receptor 4