Neisseria Meningitidis Antigen Orf1 antibody

Catalog No. ABIN125757

Product Details

Target: Neisseria Meningitidis Antigen Orf1

Reactivity: Neisseria meningitidis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Cross-Reactivity (Details): Neisseria meningitidis

Purification: Purified by ligand affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Application Details

Application Notes: Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Prochazkova, Osicka, Linhartova, Halada, Sulc, Sebo: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).

Target Details

Target: Neisseria Meningitidis Antigen Orf1

Alternative Name: Neisseria meningitidis antigen Orf1 / FrpD

Background: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD