CD16 antibody

Catalog No. ABIN126000

Product Details anti-CD16 Antibody

Target: CD16

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)

Specificity: The antibody reacts with the epitope on CD16 antigen that residing in proximity to FG loop (probably BC or C'E loop). CD16 is a low affinity receptor for aggregated IgG (FcgRIII antigen). The antibody reacts with CD16+ granulocytes.

Purification: Affinity Chromatography on Protein-A.

Sterility: 0.2 μm filtered

Immunogen: Human granulocytes

Clone: MEM-154

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 5-10 mg/mL. Positive control: PBL (peripheral blood lymphocytes). Application note: The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Immunoprecipitation. Western Blotting (Non-reducing conditions). Functional Application: The antibody MEM-154 blocks binding of human IgG to FcgRIII.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide free PBS, pH ~7.4 preserved by 0.2 μm filter sterilization.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16 (CD16 Products)

Synonyms: Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody

Background: CD16 (FcgRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgRIII is expressed in two forms FcgRIII-A and -B. FcgRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FceRI-g subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgRIII-A is associated, moreover, with FceRI-b subunit. Besides IgG, FceRI-A can be triggered also by oligomeric IgE. FcgRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.Synonyms: CD16A, FCG3, FCGR3, FCGR3A, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A

Gene ID: 2214

UniProt: P08637

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process