BCL10 antibody (pSer138)

Catalog No. ABIN126733

Product Details anti-BCL10 Antibody

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Binding Specificity: pSer138

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BCL10 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody specifically recognizes bcl-10 phosphorylated at Serine 138 at 32 kDa in Western blot.

Characteristics: Synonyms: BCL10, CIPER, CLAP, cCARMEN, mE10, c-E10, hCLAP, B-cell CLL/lymphoma 10, CED-3/ICH-1prodomain homologous E10-like regulator, CARD-containing molecule enhancingNF-kappa-B, Cellular homolog of vCARMEN, Mammalian CARD-containing adapter moleculeE10, Cellular-E10, CARD-like apoptotic protein

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to KLH

Clone: 6D3

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. Included Positive Control: Cell lysate from serum starved SW480 cells (please see: 'Protocols').
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Cell Lysate: SW 480 Untreated

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL water (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Sodium Azide / PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for BCL10

Target: BCL10 (B-Cell CLL/lymphoma 10 (BCL10))

Alternative Name: Bcl-10 (BCL10 Products)

Synonyms: CARMEN antibody, CIPER antibody, CLAP antibody, c-E10 antibody, mE10 antibody, BCL10 antibody, AI132454 antibody, BCL-10 antibody, C81403 antibody, ME10 antibody, cE10 antibody, bcl10 antibody, B-cell CLL/lymphoma 10 antibody, B-cell lymphoma/leukemia 10-like antibody, B cell leukemia/lymphoma 10 antibody, B-cell lymphoma/leukemia 10 antibody, BCL10 antibody, Bcl10 antibody, bcl10 antibody, LOC100223183 antibody

Background: Bcl-10 is an apoptosis-inducing molecule interacting with caspase 9. Bcl-10 enhances pro-caspase 9 processing and induces apoptosis through caspase 9 activation.Synonyms: B-cell CLL/lymphoma 10, BCL10, CARD-containing molecule enhancing NF-kappa-B, CARD-like apoptotic protein, CED-3/ICH-1 prodomain homologous E10-like regulator, CIPER, CLAP, Cellular homolog of vCARMEN, Cellular-E10, Mammalian CARD-containing adapter molecule E10, c-E10, cCARMEN, hCLAP, mE10

Gene ID: 8915

UniProt: O95999

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins