ERBB4 antibody (pTyr1242)

Catalog No. ABIN126796

This Mouse Monoclonal antibody specifically detects ERBB4 in WB. It exhibits reactivity toward Human.

Quick Overview for ERBB4 antibody (pTyr1242) (ABIN126796)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERBB4 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 4C6

Product Details anti-ERBB4 Antibody

Binding Specificity: pTyr1242

Specificity: This antibody specifically recognizes erbB4 phosphorylated on Tyr 1242 at 180 kDa.

Characteristics: Synonyms: p180erbB4, c-ErbB-4, HER-4, Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-likeprotein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Phosphopeptide conjugated to hemocyanin

Isotype: IgG

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive control: Cell lysate from EGF-treated MDA-MB 468 cellsFormulationLyophilized cell lysate from MDA-MB-468 cells. Serum starved cells were treated for 15 min with EGF.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose/50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquot) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: ERBB4 / HER4

Background: ErbB4/Her4 is a member of the receptor tyrosine kinase subfamily that includes EGFR, erbB2/her2, and erbB3/Her3. erbB4 is a receptor for Neuregulin 1 (NRG1) and regulates cell proliferation and differentiation. Tyr 1242 is a potential SHC binding site which leads to MAPK signalling pathway.Synonyms: HER-4, Proto-oncogene-like protein c-ErbB-4, Receptor tyrosine-protein kinase erbB-4, Tyrosine kinase-type cell surface receptor HER4, c-ErbB-4, p180erbB4

Gene ID: 2066

UniProt: Q15303

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway