Insulin Receptor antibody (Beta Chain)

Catalog No. ABIN126820

This Mouse Monoclonal antibody specifically detects Insulin Receptor in WB, EIA and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for Insulin Receptor antibody (Beta Chain) (ABIN126820)

Target: Insulin Receptor (INSR)

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 9H4

Product Details anti-Insulin Receptor Antibody

Binding Specificity: Beta Chain

Specificity: This antibody specifically recognizes the activation loop of Insulin receptor (phosphorylation-independent).

Characteristics: Synonyms: Insulin Receptor

Purification: Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated T47D cells. ELISA: 0.1 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: CD220 / INSR

Background: The insulin receptor (InsR) is a heterodimeric receptor tyrosine kinase with an extracellular alpha-chain, a transmembrane domain and an intracellular beta-chain. The insulin receptor is activated upon binding of the peptide hormone insulin, leading to autophosphorylation of tyrosine residues 1146, 1150, and 1151 in the activation loop of the beta-chain. Additional phosphorylation sites such as tyrosine residues 960, 1316, and 1322 regulate the assembly of signal transduction complexes.Synonyms: Insulin Receptor

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity