MAP2K3 antibody

Catalog No. ABIN126840

The Mouse Monoclonal anti-MAP2K3 antibody (Clone 5F7) (ABIN126840) specifically detects MAP2K3 in WB and EIA. The antibody is reactive with Human, Mouse and Dog samples.

Quick Overview for MAP2K3 antibody (ABIN126840)

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2K3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 5F7

Product Details anti-MAP2K3 Antibody

Specificity: This antibody specifically recognizes the N-terminus of MKK3.

Characteristics: Synonyms: MAPKK 3, MEK3, MKK3, MAP kinase kinase 3, Dual specificity mitogen-activated proteinkinase kinase 3

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: Cell lysate from untreated A431 cells. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MAP2K3

Target: MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3))

Alternative Name: MAP2K3

Background: MKK3 activates p38 MAP kinase by phosphorylating a Thr and Tyr residue in the activation loop. MKK3 does not phosphorylate and activate the other major MAP kinases, MAPK1/2 (erk1/2) or SAPK/JNK. Cellular stress and inflammatory cytokines activate MKK3 and lead to phosphorylation of Ser 189 and Thr 193.Synonyms: Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MEK3, MKK3

Gene ID: 5606

UniProt: P46734

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2