AKT1 antibody (pSer473) (Biotin)

Catalog No. ABIN126852

The Biotin-conjugated Mouse Monoclonal anti-AKT1 antibody (Clone 1-10E-007) (ABIN126852) specifically detects AKT1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for AKT1 antibody (pSer473) (Biotin) (ABIN126852)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This AKT1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1-10E-007

Product Details anti-AKT1 Antibody (Biotin)

Binding Specificity: pSer473

Specificity: This antibody specifically recognizes PKB phosphorylated at serine 473 in Western blot and ELISA applications.

Characteristics: Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS/0.09 % Na-Azide/PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody (aliquote in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1 / PKB

Background: The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway. Activated PKB is phosphorylated at serine 473 (mTOR/Rictor or DNA-PK) and threonine 308 (PDK1).Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase

Gene ID: 207

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect