Western Blot: 0.5 μg/mL for HRPO/ECL detectionRecommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL H2O (15 min, RT).
Buffer
1 mL2 x PBS, 0.09 % Sodium Azide, PEG and Sucrose
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C/-80 °C
Storage Comment
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: one year from despatch.
Expiry Date
12 months
Target
Topoisomerase I (TOP1)
(Topoisomerase (DNA) I (TOP1))
CG6146 antibody, Dmel\\CG6146 antibody, TOP1 antibody, TopI antibody, Topo I antibody, TopoI antibody, dTopoI antibody, l(1)G0134 antibody, l(1)G0201 antibody, l(1)G0229 antibody, l(1)G0278 antibody, l(1)top1 antibody, top1 antibody, topo I antibody, topoI antibody, DNA topoisomerase 1 beta antibody, DNA topoisomerase I alpha antibody, MGO1 antibody, MGOUN 1 antibody, MTE17.1 antibody, MTE17_1 antibody, TOP1BETA antibody, TOPOISOMERASE 1 antibody, TOPI antibody, AI467334 antibody, D130064I21Rik antibody, Top-1 antibody, Ab2-086 antibody, topi antibody, Topoisomerase 1 antibody, DNA topoisomerase I antibody, DNA topoisomerase I alpha antibody, DNA topoisomerase 1 antibody, topoisomerase (DNA) I antibody, DNA topoisomerase I, gene 1 L homeolog antibody, Top1 antibody, top1 antibody, topA antibody, ZMO1193 antibody, MRAD2831_RS63095 antibody, Lferr_0016 antibody, PC1_2015 antibody, Za10_0136 antibody, Gbro_0575 antibody, Dd586_2108 antibody, Kvar_3044 antibody, Alvin_2069 antibody, Dacet_0849 antibody, Mrub_1615 antibody, Arnit_2962 antibody, Ndas_4953 antibody, Trad_2377 antibody, Acear_1653 antibody, Mahau_0844 antibody, Mesop_4109 antibody, Thein_1586 antibody, Theth_0241 antibody, TOP1ALPHA antibody, top-1 antibody, TOP1 antibody, top1.1.L antibody
Target Type
Viral Protein
Background
Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100 kDa protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then relegated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus, and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability. DNA Topoisomerases catalyze strand breakage of DNA molecules. During DNA cleavage, a tyrosyl residue of the enzyme breaks the DNA strand by forming a covalent phosphotyrosine residue. Rejoining of the DNA strands occurs by a second transesterification reaction.Synonyms: DNA topoisomerase 1, DNA topoisomerase I, Top-1