Caspase 8 antibody

Catalog No. ABIN126934

This Mouse Monoclonal antibody specifically detects Caspase 8 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for Caspase 8 antibody (ABIN126934)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: C502S

Product Details anti-Caspase 8 Antibody

Specificity: Detects the 10 kDa small subunit of caspase-8. The antibody does not detect the full-length of Caspase-8.

Characteristics: Synonyms: CASP-8, CASP8, MCH5, CAP4, ICE-like apoptotic protease 5, MORT1-associated CED-3homolog, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, Apoptotic cysteineprotease, Apoptotic protease Mch-5

Purification: Affinity Chromatography.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. Immunoprecipitation: 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS containing 50 % Glycerol, 1 % BSA, and 0.02 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Caspase-8, a member of the caspase-family of proteases, plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases, such as caspase-3, -6, -7 and a death agonist member of the Bcl-2/Bcl-xL family, Bid, leading to apoptosis.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog

Gene ID: 841

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades