ROCK1 antibody (Cleavage Site)

Catalog No. ABIN126943

This Mouse Monoclonal antibody specifically detects ROCK1 in WB. It exhibits reactivity toward Human.

Quick Overview for ROCK1 antibody (Cleavage Site) (ABIN126943)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 12M03

Product Details anti-ROCK1 Antibody

Binding Specificity: Cleavage Site

Specificity: The antibody has been selected for its ability to recognize the cleaved ROCK-1 in Western blotting. It does not recognize the full length protein.

Characteristics: Synonyms: ROCK-1, p160ROCK, NY-REN-35, Rho-associated protein kinase 1, Renal carcinoma antigenNY-REN-35, Rho-associated, coiled-coil-containing protein kinase 1

Purification: Protein G Chromatography.

Immunogen: Synthetic peptide corresponding to the Caspase-3 cleavage site

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1-4 μg/mL. Jurkat cell lysate treated with Camptothecin (2μM for 6-8 hours) can be used as a PositiveControl.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol, 1 % BSA and 0.02 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: ROCK1

Background: ROCK-1 (Rho-associated coiled coil-containing protein kinase 1) has been found to be a new caspase-3 substrate. ROCK-1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain. During apoptosis, ROCK-1 is cleaved by caspase-3 at the conserved DETD1113/G sequence and its C-terminal inhibitory domain is removed, resulting in deregulated and constitutive kinase activity. The caspase-3 mediated cleavage and activation of ROCK-1 induces phosphorylation of MLC and membrane blebbing, the early events in the execution phase of apoptosis.Synonyms: NY-REN-35, ROCK-1, Renal carcinoma antigen NY-REN-35, Rho-associated, Rho-associated protein kinase 1, coiled-coil-containing protein kinase 1, p160ROCK

Gene ID: 6093

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling