Caspase 8 antibody

Catalog No. ABIN126965

The Rabbit Polyclonal anti-Caspase 8 antibody is suitable to detect Caspase 8 in samples from Human, Mouse, Rat, Cow and Monkey. It has been validated for WB.

Quick Overview for Caspase 8 antibody (ABIN126965)

Target: Caspase 8 (CASP8)

Reactivity: Human, Mouse, Rat, Cow, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 8 Antibody

Specificity: The immunoaffinity purified anti-Caspase 8 antibody detects the full length (55 kDa), intermediate cleavage fragments (26-31 kDa) and cleaved large fragment (18 kDa) of Caspase-8.

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 392 of Mouse Caspase-8.

Application Details

Application Notes: Western Blot (0.5-4 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA as stabilizer and 0.01 % Thimerosal as preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: Caspase-8

Background: Caspase family of cysteine proteases has been shown to play a key role in apoptosis. Caspase-8 is a 55 kDa cytosolic protein that is synthesized as an inactive pro-enzyme. Activation of caspase-8 involves a two-step proteolysis: the cleavage of caspase-8 to generate a 43 and a 12 kDa fragment which is further processed to 10 kDa. The p43 is then cleaved to yield p26 and the release of the active site containing p18.Synonyms: Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, CASP-8, CASP8, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, ICE-like apoptotic protease 5, MCH5, MORT1-associated CED-3 homolog

Gene ID: 841

NCBI Accession: NP_001073593

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades