SMAD5 antibody

Catalog No. ABIN127143

Product Details anti-SMAD5 Antibody

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody recognizes ~60 kDa Smad5.

Purification: Affinity Purified

Application Details

Application Notes: Western Blot: 1-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD5 (SMAD5 Products)

Synonyms: DWFC antibody, JV5-1 antibody, MADH5 antibody, Madh5 antibody, SBN antibody, fb67b04 antibody, mad antibody, madh5 antibody, sb:cb400 antibody, smd5 antibody, wu:fb67b04 antibody, SMAD5 antibody, DKFZp468M0619 antibody, 1110051M15Rik antibody, AI451355 antibody, Dwf-C antibody, MusMLP antibody, SMAD family member 5 antibody, SMAD5 antibody, Smad5 antibody, smad5 antibody

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: JV5-1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, Mothers against decapentaplegic homolog 5, SMAD 5, SMAD family member 5, SMAD-5

Gene ID: 4090

NCBI Accession: NP_001001419

UniProt: Q99717