BUBR1 Kinase Kinase (AA 1-350) antibody

Catalog No. ABIN129516

Product Details

Target: BUBR1 Kinase Kinase

Binding Specificity: AA 1-350

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
Immunogentype:Recombinant

Clone: 8G1

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.  Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein.  The use of HeLa whole cell lysates is recommended as a positive control.  For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8.  Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Lázaro-Diéguez, Ispolatov, Müsch: "Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum." in: Molecular biology of the cell, Vol. 26, Issue 7, pp. 1286-95, (2016) (PubMed).

Target Details

Target: BUBR1 Kinase Kinase

Background: BUBR1 Kinase (also called Mitotic checkpoint serine/threonine-protein kinase, BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L and SSK1) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores.  This protein is highly expressed in thymus followed by spleen.  Defects in BUB1B are associated with tumor formation.
Synonyms: Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody

Gene ID: 701

UniProt: O60566