PCNA antibody (AA 50-75)

Catalog No. ABIN129658

Supplier Product No.: 600-401-887

This Rabbit Polyclonal antibody specifically detects PCNA in WB, ELISA and IP. It exhibits reactivity toward Microalgae (Isochrysis galbana).

Quick Overview for PCNA antibody (AA 50-75) (ABIN129658)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Microalgae (Isochrysis galbana)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-PCNA Antibody

Binding Specificity: AA 50-75

Supplier Product No.: 600-401-887

Supplier: Rockland

Purpose: PCNA Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against algal PCNA.

Characteristics: Synonyms: rabbit anti-PCNA antibody, Proliferating cell nuclear antigen

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 50-75 of Isochrysis galbana PCNA.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~29 kDa in size corresponding to PCNA by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:5,000 - 1:32,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.42 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: Background: PCNA, or Proliferating Cell Nuclear Antigen, is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.  PCNA is a nuclear protein.

Gene ID: 7715035

UniProt: Q9LD36

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy