ECT2 antibody (pThr790)

Catalog No. ABIN129688

Product Details anti-ECT2 Antibody

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Binding Specificity: AA 785-795, pThr790

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Zebrafish (Brachydanio rerio), Chimpanzee, Chicken, Dog (Canine)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 785-795 of human ECT2 protein.

Isotype: IgG

Application Details

Application Notes: This affinity-purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 101 kDa in size by western blotting in the appropriate cell lysate or extract.  Less than 2% reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is believed to be phospho specific for ECT2 phosphorylated at the pT790 residue.  Experiments to generate more conclusive evidence are currently under preparation.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for ECT2

Target: ECT2 (Epithelial Cell Transforming Sequence 2 Oncogene (ECT2))

Alternative Name: ECT2 (ECT2 Products)

Synonyms: ECT2 antibody, arhgef31 antibody, xect2 antibody, AI528536 antibody, mKIAA4037 antibody, ARHGEF31 antibody, ect2 antibody, epithelial cell transforming 2 antibody, ect2 oncogene antibody, epithelial cell transforming 2 S homeolog antibody, ECT2 antibody, ect2 antibody, Ect2 antibody, ect2.S antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. ECT2, also known as epithelial cell transforming sequence 2 oncogene, was originally isolated as a transforming gene from epithelial cells. ECT2 catalyzes guanine nucleotide exchange on the small GTPases, RhoA, Rac1, and Cdc42.  ECT2 may be phos-phorylated during G₂ and M phases, and phosphorylation may be required for its exchange activity. Unlike other known guanine nucleotide exchange factors for Rho GTPases, ECT2 exhibits nuclear localization in interphase, spreads throughout the cytoplasm in prometaphase, and is condensed in the midbody during cytokinesis. Expression of dominant-negative ECT2 or microinjection of affinity-purified anti-ECT2 antibody into interphase cells strongly inhibits cytokinesis. These results suggest that ECT2 is an important link between the cell cycle machinery and Rho signaling pathways involved in the regulation of cell division.  Phosphorylation at T790 or S375 significantly affects the catalytic activity of ECT2.
Synonyms: ECT 2 antibody, ECT2 protein antibody, Epithelial cell transforming sequence 2 antibody

Gene ID: 1894, 21735572

UniProt: Q9H8V3

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization