CD5 antibody (Biotin)

Catalog No. ABIN133206

Product Details anti-CD5 Antibody (Biotin)

Target: CD5

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: Rat CD5

Immunogen: Rat thymocyte glycoproteins, depleted of LC-A, W3/13 antigen and Thy 1. . Fusion Partner: P3-NSI-I-Ag4(NS1/1) . Immunocyte Donor: BALB/c spleen

Clone: OX-19

Isotype: IgG1

Application Details

Application Notes: This clone is reported to work with frozen and paraffin sections (4).

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/ml

Buffer: Biotin conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from ascitic fluid via Protein G Chromatography)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Synonyms: CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background: Anti-rat CD5 monoclonal antibody recognizes a determinant expressed on all rat thymocytes and peripheral T lymphocytes. It has been determined that there are 15,000 antigenic sites for ABIN133206per cell on thymocytes and 30,000 on peripheral T cells. This antibody does not bind to B cells, macrophages, NK cells or mast cells (2). Also, no cross- reactivity has been detected with non-lymphoid cells in intestine, thymus, spleen or lymph node (2). The antigen recognized by ABIN133206is a surface glycoprotein of Mr 69,000 (2), and is most likely the homologue of the mouse Ly 1 and human CD5 antigen. The antigen has also been shown to have an important functional role in T cell activation and differentiation indicating a use for this antibody in related studies. The antibody may be employed to label cells with anti-mouse Ig conjugated to fluorescein, peroxidase or 125I and has been successfully used in labelling of cryostat sections (3). This clone is reported to work with frozen and paraffin sections (4).