CD5 antibody (FITC)

Catalog No. ABIN133208

Product Details anti-CD5 Antibody (FITC)

Target: CD5

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: Rat CD5

Immunogen: Rat thymocyte glycoproteins, depleted of LC-A, W3/13 antigen and Thy 1. . Fusion Partner: P3-NSI-I-Ag4(NS1/1) . Immunocyte Donor: BALB/c spleen

Clone: OX-19

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Concentration: 0.1 g per 10 cells

Storage: 4 °C

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Synonyms: CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background: Anti-rat CD5 monoclonal antibody recognizes a determinant expressed on all rat thymocytes and peripheral T lymphocytes. It has been determined that there are 15,000 antigenic sites for ABIN133208per cell on thymocytes and 30,000 on peripheral T cells. This antibody does not bind to B cells, macrophages, NK cells or mast cells (2). Also, no cross- reactivity has been detected with non-lymphoid cells in intestine, thymus, spleen or lymph node (2). The antigen recognized by ABIN133208is a surface glycoprotein of Mr 69,000 (2), and is most likely the homologue of the mouse Ly 1 and human CD5 antigen. The antigen has also been shown to have an important functional role in T cell activation and differentiation indicating a use for this antibody in related studies. The antibody may be employed to label cells with anti-mouse Ig conjugated to fluorescein, peroxidase or 125I and has been successfully used in labelling of cryostat sections (3).