RT1.D antibody
Reactivity: Rat
FACS
Host: Mouse
Monoclonal
MRC OX-17
unconjugated
-
- Target
- RT1.D
- Reactivity
- Rat
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS)
- Specificity
- Rat RT1.D (Rat Ia-E)
- Purification
- Purified from ascitic fluid via Protein G Chromatography).
- Immunogen
- Rat spleen membrane glycoproteins depleted of Ia-A antigens. . Fusion Partner: X63 Ag8.653 . Immunocyte Donor: BALB/c spleen
- Clone
- MRC OX-17
- Isotype
- IgG1
-
-
- Comment
-
Hybridoma Production: Immunization: Immunogen: Rat spleen membrane glycoproteins depleted of Ia-A antigens. Immunocyte Donor: BALB/c spleen. Fusion Partner: X63 Ag8.653.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/ml
- Buffer
- PBS + 0.02% NaN3
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
-
- Target
- RT1.D
- Background
- Anti-rat RT1.D monoclonal antibody recognizes a monomorphic determinant on the chain of the rat Ia antigen and appears to be the rat homologue of mouse Ia-E. It recognizes the rat Ia product present on B, but not T cells from lymph node or thoracic duct lymph. It does not bind to thymocytes or erythrocytes. The antibody does not cross-react with rat Ia-A or mouse Ia-E antigen, but rabbit antibody raised against the antibody affinity column-purified MRC OX-17 antigen cross-reacted on tissues of mice expressing Ia-E mouse antigen but not on those mouse strains that were Ia-E antigen negative (2).
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