CD45 antibody, L-CA antibody, Lca antibody, RT7 antibody, T200 antibody, protein tyrosine phosphatase, receptor type, C antibody, Ptprc antibody
Background
Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes gives one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa and B cells one broad band at 240 kDa. T helper and T cytotoxic cell subsets show the same 4 bands with some differences in the proportions of each. ABIN133289reacts with the high molecular weight form of the leukocyte common antigen (2) which includes the 240 kDa form on B cells as well as a subfraction of the 190, 200 and 220 kDa forms, but not with the 180 kDa form of T cells or thymocytes. (5) Thus, ABIN133289reacts with B cells, cytotoxic T cells and helper cells for T cell responses but not with thymocytes or helper T cells for in vivo antibody responses. (5) Consequently, this antibody can be used to identify B cells, all CD8+ve T cells, 75% CD4+ve T cells and 50% bone marrow cells. This, antibody also divides rat CD4+ve T cells into two phenotypically distinct populations differing in their helper activities (6,7).