Dendritic Cells antibody (FITC)
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- Target
- Dendritic Cells
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- Rat lymphoid and non-lymphoid dendritic cells
- Purification
- Purified from ascites via Protein G Chromatography
- Clone
- OX-62
- Isotype
- IgG1
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.1 mg/ml
- Buffer
- FITC conjugated Ig buffered in PBS, 0.02% NaN and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Dendritic Cells
- Alternative Name
- Dendritic Cell
- Background
- ABIN133165 (OX-62) monoclonal antibody was raised against density gradient-enriched rat veiled (dendritic) cells obtained from lymph. This antibody only labeled cells characteristic of veiled cells the antigen recognized by ABIN133165 is expressed at low to moderate levels by a subset of spleen, thymus and bone marrow dendritic cells. In lymphoid tissues, the labeling correlated with dendritic cells (DC), however in skin, MHC Class II cells were ABIN133165 , while other CD3 cells with dendritic morphology were strongly stained with ABIN133165. Therefore, it seems that this antibody may be restricted to DC and probably T cells. (This has since been contradicted by the fact that the CD3 cells with dendritic morphology were confirmed to stain negative for ABIN133204 (OX-19), and are therefore not T cells.) The ABIN133165 antigen has the biochemical characteristics of an integrin (ABIN133165 recognizes the -like subunit) as determined by SDS-PAGE. This monoclonal antibody is useful for cell purification, delineating lineage relationships among cells with dendritic morphology and for molecular studies. The OX-62 antigen is not essential for T cell activation. The ABIN133165 clone has been use in flow cytometry, immunohistochemistry, immunoprecipitation and cell surface radioiodination.
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