Sca-1/Ly-6A/E antibody

Catalog No. ABIN133910

Product Details anti-Sca-1/Ly-6A/E Antibody

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Mouse Sca-1

Purification: Purified from ascitic fluid via Protein G Chromatography

Isotype: IgG2b

Application Details

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/ml

Buffer: Purified Ig buffered in PBS and 0.02% NaN3 (Purified from ascites via Protein G Chromatography).

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Sca-1/Ly-6A/E antibody (ABIN133910)

Sin, Puccini, Huang, Konstandin, Gilbert, Sussman, Gottlieb, Feuer: "The impact of juvenile coxsackievirus infection on cardiac progenitor cells and postnatal heart development." in: PLoS pathogens, Vol. 10, Issue 7, pp. e1004249, (2015) (PubMed).

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Sca-1 (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Background: Monoclonal antibody recognizes Sca-1 (Ly-6A.2/6E.1), a cell surface antigen used in the identification of hematopoietic stem cells. It is a member of the Ly-6 antigen family. The Thy-1 lo , Lin (lineage-negative, not expressing B220, Gr-1, Mac-1, CD4 or CD8), Sca-1 population of bone marrow cells are highly purified, perhaps homogenous, pluripotent stem cells. This antigen is also present on various other tissues. Specific staining of the parenchymal cells can be demonstrated in thymus, spleen and kidney where as only vasculature reacts with anti-Sca- 1 in brain, heart and liver (and possibly in lung). Also, Sca-1 is a T cell activation antigen, as surface expression of the antigen increases upon Con A activation of T lymphocytes. Sca-1 appears to have a molecular mass of 8 kDa under non-reducing conditions and 18 kDa under reducing conditions, indicating the presence of intra-chain disulfide bonds.

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation