H-2Kd/H-2Dd antibody (Biotin)

Catalog No. ABIN135418

Product Details

Target: H-2Kd/H-2Dd

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Specificity: Mouse H-2Kd/H-2Dd

Characteristics: Mouse Anti-Mouse H-2Kd/H-2Dd-BIOT

Immunogen: BDF1 mouse splenocytes

Clone: 34-1-2S

Isotype: IgG2a

Application Details

Application Notes:

• Applications: FC - Quality tested , ICC - Reported in literature , EM - Reported in literature , IP - Reported in literature , CMCD - Reported in literature , Block - Reported in literature , Adhesion - Reported in literature
• Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Comment:

Blocking Cell Adhesion, Complement-mediated cytotoxicity

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: 0.5 mg in 1.0 mL of PBS/Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Abel, Goto, Magnuson, Abraham, Ramanathan, Hotaling, Alaniz, Kumar-Sinha, Dziubinski, Urs, Wang, Shi, Waghray, Ljungman, Crawford, Simeone: "HNF1A is a novel oncogene that regulates human pancreatic cancer stem cell properties." in: eLife, Vol. 7, (2019) (PubMed).

Pal, Dziubinski, Di Magliano, Simeone, Owens, Thomas, Peterson, Potu, Talpaz, Donato: "Usp9x Promotes Survival in Human Pancreatic Cancer and Its Inhibition Suppresses Pancreatic Ductal Adenocarcinoma In Vivo Tumor Growth." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 2, pp. 152-164, (2018) (PubMed).

Chen, Fisher, Signs, Molina, Shenoy, Lopez, Baker, Koomen, Chen, Gittleman, Barnholtz-Sloan, Berg, Appelman, Huang: "Inhibition of PI3K/Akt/mTOR signaling in PI3KR2-overexpressing colon cancer stem cells reduces tumor growth due to apoptosis." in: Oncotarget, Vol. 8, Issue 31, pp. 50476-50488, (2017) (PubMed).

Target Details

Target: H-2Kd/H-2Dd

Background: The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic chains noncovalently associated with the invariant 2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.