Monocyte/Granulocyte antibody (PE)

Catalog No. ABIN135483

Product Details anti-Monocyte/Granulocyte Antibody (PE)

Target: Monocyte/Granulocyte

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Monocyte/Granulocyte antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: Porcine monocytes & granulocytes, Mr 230 kDa - macrophages

Characteristics: Mouse Anti-Porcine Monocyte/Granulocyte-PE

Purification: Rabbit Anti-Cofilin (Phospho-Ser3) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Fresh dd miniature swine thymocytes

Clone: 74-22-15

Isotype: IgG1

Application Details

Application Notes:

• Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , ELISA - Reported in literature , Sep - Reported in literature
• Working Dilutions: Flow Cytometry FITC, BIOT and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Sample Volume: 1 mL

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References for anti-Monocyte/Granulocyte antibody (PE) (ABIN135483)

Sauter, Waddell, Lisowski, Young, Lefevre, Davis, Clohisey, McCulloch, Magowan, Mabbott, Summers, Hume: "Macrophage colony-stimulating factor (CSF1) controls monocyte production and maturation and the steady-state size of the liver in pigs." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 311, Issue 3, pp. G533-47, (2017) (PubMed).

Guo, Fu, Wang, Lu, Wei, Tang, Fan, Liu, Zhang, Zhang, Huang, Liu, Li, Wu, Liu: "A porcine circovirus type 2 (PCV2) mutant with 234 amino acids in capsid protein showed more virulence in vivo, compared with classical PCV2a/b strain." in: PLoS ONE, Vol. 7, Issue 7, pp. e41463, (2012) (PubMed).

Hosoya, Villena, Shimazu, Tohno, Fujie, Chiba, Shimosato, Aso, Suda, Kawai, Saito, Alvarez, Ikegami, Itoh, Kitazawa: "Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells." in: Veterinary research, Vol. 42, pp. 111, (2011) (PubMed).

Fort, Fernandes, Nofrarias, Díaz, Sibila, Pujols, Mateu, Segalés: "Development of cell-mediated immunity to porcine circovirus type 2 (PCV2) in caesarean-derived, colostrum-deprived piglets." in: Veterinary immunology and immunopathology, Vol. 129, Issue 1-2, pp. 101-7, (2009) (PubMed).

Target Details for Monocyte/Granulocyte

Target: Monocyte/Granulocyte

Abstract: Monocyte/Granulocyte Products