CD28 antibody
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- Target See all CD28 Antibodies
- CD28
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Reactivity
- Chicken
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD28 antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- Chicken/Turkey CD28, Mr 40-44 kDa
- Characteristics
- Mouse Anti-Chicken CD28-UNLB
- Purification
- Purified
- Immunogen
- Chicken peripheral blood lymphocytes
- Clone
- AV7
- Isotype
- IgG1
- Top Product
- Discover our top product CD28 Primary Antibody
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- Application Notes
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , ICC - Reported in literature , IP - Reported in literature , Costim - Reported in literature
- Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
- Comment
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In vitro T-cell costimulation
- Sample Volume
- 1 mL
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- 0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
- Preservative
- Without preservative
- Handling Advice
- Each reagent is stable for the period shown on the bottle label if stored as directed.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C
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Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C." in: Veterinary immunology and immunopathology, (2010) (PubMed).
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Pathogenic and immunogenic responses in turkeys following in ovo exposure to avian metapneumovirus subtype C." in: Veterinary immunology and immunopathology, (2010) (PubMed).
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- Target
- CD28
- Alternative Name
- CD28 (CD28 Products)
- Synonyms
- Tp44 antibody, CD28RNA antibody, cd28 antibody, CD28 molecule antibody, CD28 antigen antibody, Cd28 molecule antibody, CD28 molecule L homeolog antibody, CD28 antibody, Cd28 antibody, cd28.L antibody
- Background
- Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V1- and V2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.
- Pathways
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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