SPRY1 antibody (AA 221-319)
-
- Target See all SPRY1 Antibodies
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
-
Binding Specificity
- AA 221-319
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This SPRY1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Pig,Horse,Chicken
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Sprouty1
- Isotype
- IgG
- Top Product
- Discover our top product SPRY1 Primary Antibody
-
-
- Application Notes
-
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
-
- Target
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- Alternative Name
- Sprouty1 (SPRY1 Products)
- Synonyms
- hSPRY1 antibody, SPRY1 antibody, LOC100223875 antibody, hspry1 antibody, xspry1 antibody, sprouty antibody, sprouty1 antibody, sprouty-1 antibody, Xtsprouty1 antibody, spry1a antibody, spry1b antibody, sprouty RTK signaling antagonist 1 antibody, sprouty RTK signaling antagonist 1 L homeolog antibody, sprouty RTK signaling antagonist 1 S homeolog antibody, sprouty homolog 1 (Drosophila) antibody, SPRY1 antibody, Spry1 antibody, spry1 antibody, spry1.L antibody, spry1.S antibody
- Background
-
Synonyms: Sprouty 1, Sprouty-1, hSPRY1, Protein sprouty homolog 1, Sprouty homolog 1 antagonist of FGF signaling, Sprouty homolog 1, Spry-1, Spry1, SPY1_HUMAN.
Background: Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well-conserved cysteine-rich C-terminal domain with their Drosophila counterparts. Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues. Upon stimulation, a subset is recruited to the leading edge of the plasma membrane. Sprouty 2 can associate with c-Cbl, a down regulator of RTK signaling, and inhibits the activities of several growth factors. Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth. The well-conserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 co-localization with microtubules and translocation to membrane ruffles. In addition, the C-terminus is required for the inhibition of cell migration and proliferation. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.
- Pathways
- EGFR Signaling Pathway, EGFR Downregulation
-