PPP1R10 antibody (AA 151-250)
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- Target See all PPP1R10 Antibodies
- PPP1R10 (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 10 (PPP1R10))
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Binding Specificity
- AA 151-250
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PPP1R10 antibody is un-conjugated
- Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Dog,Cow,Sheep,Pig
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human PNUTS/PPP1R10
- Isotype
- IgG
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- Application Notes
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- PPP1R10 (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 10 (PPP1R10))
- Alternative Name
- PNUTS/PPP1R10 (PPP1R10 Products)
- Synonyms
- CAT53 antibody, FB19 antibody, PNUTS antibody, PP1R10 antibody, R111 antibody, p99 antibody, Fb19 antibody, Pnuts antibody, 2610025H06Rik antibody, Cat53 antibody, D17Ertd808e antibody, CHUNP6885 antibody, fe15d12 antibody, fk54b08 antibody, ppp1r10l antibody, wu:fe15d12 antibody, wu:fk54b08 antibody, protein phosphatase 1 regulatory subunit 10 antibody, protein phosphatase 1, regulatory subunit 10 antibody, protein phosphatase 1 regulatory subunit 10 S homeolog antibody, PPP1R10 antibody, Ppp1r10 antibody, ppp1r10.S antibody, ppp1r10 antibody
- Background
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Synonyms: CAT 53, CAT53, FB 19, FB19, FB19 protein, MHC class I region proline rich protein CAT53, p99, Phosphatase 1 nuclear targeting subunit, Phosphatase nuclear targeting subunit, PNUTS, PP1 binding protein of 114 kDa, PP1R10, Protein phosphatase 1 regulatory inhibitor subunit 10, Protein phosphatase 1 regulatory subunit 10, Serine/threonine protein phosphatase 1 regulatory subunit 10, PP1RA_HUMAN.
Background: Eukaryotic protein phosphorylation and dephosphorylation on serine and threonine residues regulates numerous cell functions, including division, homeostasis and apoptosis. A group of proteins that play a major role in this process are the serine/threonine protein phosphatases. Protein phosphatase (PP) holoenzyme is a trimeric complex that contains a regulatory subunit, a variable subunit and a catalytic subunit. Families of PP catalytic subunits include PP1, PP2A, PP2B, PP2C, PPX and PP5. Regulatory subunits include nuclear inhibitor of PP1 (NIPP1), PP1 nuclear targeting subunit (PNUTS), PP2A-A, PP2A-B, PP2A-B56, PP2A-C, PP2B-B and PR48. PNUTS, also designated CAT53 or FB19, is encoded by the gene PPP1R10. PNUTS acts as an inhibitor for the phosphatase activity of PP1 Alpha and PP1 Gamma. It is a nuclear protein primarily detected in nucleoplasmic bodies and within nucleoli. PNUTS expression levels are highest in brain, heart, lung, placenta, liver, kidney, pancreas and skeletal muscle.
- Pathways
- Protein targeting to Nucleus
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