MYF5 antibody (pSer49)

Catalog No. ABIN1386790

This Rabbit Polyclonal antibody specifically detects MYF5 in WB, IF (cc), IF (p), IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MYF5 antibody (pSer49) (ABIN1386790)

Target: MYF5 (Myogenic Factor 5 (MYF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MYF5 Antibody

Binding Specificity: pSer49

Purpose: phospho-MYF5 (phospho Ser49) Polyclonal Antibody

Predicted Reactivity: Human,Mouse,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MYF5 around the phosphorylation site of Ser49

Isotype: IgG

Application Details

Application Notes: WB(1:300-5000),

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MYF5

Target: MYF5 (Myogenic Factor 5 (MYF5))

Alternative Name: Myf5

Background:

Synonyms: Myf5 phospho S49, Myf5 phospho Ser49, p-Myf5phospho S49, p-Myf5phospho Ser49, Myf-5 bHLHc2, Class C basic helix loop helix protein 2, Class C basic helix-loop-helix protein 2, Myf 5, Myf-5, Myf5, MYF5_HUMAN, Myogenic factor 5.

Background: Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.

Gene ID: 4617

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development