Mycobacterium Tuberculosis Ag85 antibody

Catalog No. ABIN1387337

Product Details

Target: Mycobacterium Tuberculosis Ag85

Reactivity: Mycobacterium tuberculosis

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: This antibody will recognize Mycobacterium tuberculosis Ag85A and Ag85B

Cross-Reactivity: Bacteria

Cross-Reactivity (Details): Mycobacteriumtuberculosis

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Mycobacterium tuberculosis Ag85B

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Mycobacterium Tuberculosis Ag85

Alternative Name: Mycobacterium Tuberculosis Ag85B

Background:

Synonyms: Antigen 85 complex B, 30 kDa extracellular protein, Mycobacterium, Mycolyl transferase 85B, fbpB, Antigen 85-B, 85B, Ag85B, Extracellular alpha-antigen, Fibronectin-binding protein B, DGAT, Acyl-CoA:diacylglycerol acyltransferase, Ag85A, fbpA, 85A

Background: The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM.

Gene ID: 885785, 886132

UniProt: P9WQP1, P9WQP3