Otx1 + Otx2 (AA 21-120) antibody

Catalog No. ABIN1387702

Product Details

Target: Otx1 + Otx2

Binding Specificity: AA 21-120

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Specificity: This has 79 % sequence similarty with CRX, which is specific to Retinal tissue.

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human OTX1 + OTX2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References

Wang, Wang, Xie, Wang: "OTX2 impedes self-renewal of porcine iPS cells through downregulation of NANOG expression." in: Cell death discovery, Vol. 2, pp. 16090, (2016) (PubMed).

Target Details

Target: Otx1 + Otx2

Background:

Synonyms: FLJ38361, Homeobox protein OTX 1, Homeobox protein OTX1, Homeobox protein OTX2, MCOPS 5, MCOPS5, MGC15736, MGC45000, Orthodenticle 1, Orthodenticle 2, Orthodenticle homeobox 1, Orthodenticle homeobox 2, Orthodenticle homolog 1, Orthodenticle homolog 2 Drosophila, Orthodenticle homolog 2, Orthodenticle1, Orthodenticle2, Otx 1, Otx 2, otx1, OTX1_HUMAN, otx2, OTX2_HUMAN.

Background: Transcription factors, OTX1 and OTX2, are two murine homologs of the Drosophila orthodenticle (OTD), show a limited amino acid sequence divergence. OTX1 and OTX2 play an important role during early and later events required for proper brain development in that they are involved in the processes of induction, specification and regionalization of the brain. OTX1 is involved in corticogenesis, sensory organ development and pituitary functions, while OTX2 is necessary earlier in development, for the correct anterior neural plate specification and organization of the primitive streak. OTX2 is also required in the early specification of the neuroectoderm, which is destined to become the fore-midbrain, and both OTX1 and OTX2 co-operate in patterning the developing brain through a dosage-dependent mechanism. A molecular mechanism depending on a precise threshold of OTX proteins is necessary for the correct positioning of the isthmic region and for anterior brain patterning. The genes which encode OTX1 and OTX2 map to human chromosomes 2p13 and 14q21-q22, respectively.

Gene ID: 5015, 5013