BDNF antibody (AA 151-247)

Catalog No. ABIN1387788

This anti-BDNF antibody is a Rabbit Polyclonal antibody detecting BDNF in WB, ELISA, ICC, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 6+ publications.

Quick Overview for BDNF antibody (AA 151-247) (ABIN1387788)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BDNF Antibody

Binding Specificity: AA 151-247

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human BDNF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-BDNF antibody (ABIN1387788)

Nader, Ateyya, El-Shafey, El-Sherbeeny et al.: "Sitagliptin enhances the neuroprotective effect of pregabalin against pentylenetetrazole-induced acute epileptogenesis in mice: Implication of oxidative, inflammatory, apoptotic and autophagy ..." in: Neurochemistry international, (2017) (PubMed).

Xing, Wen, Li, Xia: "Non-viral liposome-mediated transfer of brain-derived neurotrophic factor across the blood-brain barrier." in: Neural regeneration research, Vol. 11, Issue 4, pp. 617-22, (2016) (PubMed).

Zabłocka, Mitkiewicz, Macała, Janusz: "Neurotrophic Activity of Cultured Cell Line U87 is Up-Regulated by Proline-Rich Polypeptide Complex and Its Constituent Nonapeptide." in: Cellular and molecular neurobiology, Vol. 35, Issue 7, pp. 977-86, (2016) (PubMed).

Zhao, Li, Wei, Savage, Zhou, Ma: "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." in: Neurobiology of disease, Vol. 68, pp. 145-55, (2014) (PubMed).

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background:

Synonyms: ANON2, BULN2, Brain-derived neurotrophic factor, BDNF, Abrineurin

Background: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

Gene ID: 627

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity