NSF antibody (AA 151-250) (AbBy Fluor® 555)
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- Target See all NSF Antibodies
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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Binding Specificity
- AA 151-250
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NSF antibody is conjugated to AbBy Fluor® 555
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Rabbit,Zebrafish
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human NSF
- Isotype
- IgG
- Top Product
- Discover our top product NSF Primary Antibody
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- Alternative Name
- NSF (NSF Products)
- Synonyms
- SKD2 antibody, AI316878 antibody, AU020090 antibody, AU067812 antibody, NSF antibody, 18.m06598 antibody, nsf antibody, si:bz18k17.1 antibody, wu:fj33g11 antibody, N-ethylmaleimide sensitive factor antibody, T1J1.4 antibody, T1J1_4 antibody, NEM-sensitive fusion protein antibody, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antibody, N-ethylmaleimide sensitive fusion protein antibody, N-ethylmaleimide-sensitive factor antibody, N-ethylmaleimide sensitive factor antibody, N-ethylmaleimide-sensitive fusion protein, putative antibody, N-ethylmaleimide sensitive factor L homeolog antibody, vesicle-fusing ATPase antibody, N-ethylmaleimide-sensitive factor a antibody, AAA-type ATPase family protein antibody, NSF antibody, Nsf antibody, nsf antibody, BBOV_II007200 antibody, TGME49_318510 antibody, nsf.L antibody, LOC100180685 antibody, LOC9310886 antibody, nsfa antibody
- Background
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Synonyms: N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor, N ethylmaleimide sensitive factor like protein, N ethylmaleimide sensitive fusion protein, N ethylmaleimide sensitive fusion protein, N-ethylmaleimide-sensitive fusion protein, NEM sensitive fusion protein, NEM sensitive fusion protein, NEM-sensitive fusion protein, NSF, NSF_HUMAN, SKD 2, SKD2, SKD2, Vesicle fusing ATPase, Vesicle fusing ATPase, Vesicle-fusing ATPase, Vesicular fusion protein NSF, Vesicular fusion protein NSF, Vesicular-fusion protein NSF.
Background: Several protein-protein interactions are essential to membrane fusion during endocytosis. Membrane fusion requires interaction among SNARE1 proteins associated with both donor and acceptor membranes (1,2). Following membrane fusion, the -SNAP cytoplasmic adapter protein binds to the SNARE complex. N-ethylmaleimide-sensitive factor (NSF), a hexameric ATPase, then associates with the -SNAP/SNARE complex to mediate SNARE disassembly during membrane fusion (3,4). The ATPase activity of NSF induces a conformational change in the -SNAP/SNARE complex that leads to its dissociation from the membrane, membrane fusion and eventual recycling of the SNARE complex for subsequent membrane fusion (3,4).
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