Histone H2A.X Ubiquityl (ubLys119) antibody (Alexa Fluor 647)
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- Target
- Histone H2A.X Ubiquityl
- Binding Specificity
- ubLys119
- Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Alexa Fluor 647
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Application
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Specificity
- This modification site is homologous to that of Lys119 in Rat.
- Cross-Reactivity
- Human, Mouse, Rat
- Predicted Reactivity
- Cow,Pig,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from human Ubiquityl Histone H2A.X (Lys119)
- Isotype
- IgG
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-
- Application Notes
-
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- Histone H2A.X Ubiquityl
- Synonyms
- H2A.X antibody, H2A/X antibody, H2AX antibody, RGD1566119 antibody, H2A histone family member X antibody, H2A histone family, member X antibody, H2AFX antibody, H2afx antibody
- Background
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Synonyms: H2AX, H2A.X, H2A/X, Histone H2AX, Histone H2A.X, H2AFX
Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
- Gene ID
- 3014
- UniProt
- P16104
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