VGLL2 antibody (HRP)

Catalog No. ABIN1419190

Product Details anti-VGLL2 Antibody (HRP)

Target: VGLL2 (Vestigial Like 2 (VGLL2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VGLL2 antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VGLL2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for VGLL2

Target: VGLL2 (Vestigial Like 2 (VGLL2))

Alternative Name: VGLL2 (VGLL2 Products)

Synonyms: VGL2 antibody, VITO1 antibody, vgll2a antibody, MGC114146 antibody, zgc:114146 antibody, C130057C21Rik antibody, VITO-1 antibody, Vito1 antibody, vgl-2 antibody, zgc:110098 antibody, vestigial like family member 2 antibody, vestigial-like family member 2 antibody, vestigial-like family member 2a antibody, vestigial like family member 2 L homeolog antibody, vestigial-like family member 2b antibody, VGLL2 antibody, Vgll2 antibody, vgll2a antibody, vgll2.L antibody, vgll2b antibody

Background:

Synonyms: Protein VITO1, Transcription cofactor vestigial like 2, Transcription cofactor vestigial like protein 2, Transcription cofactor vestigial-like protein 2, Vestigial like 2, Vestigial like 2 Drosophila, Vgl-2, VGL2, VGLL 2, Vgll2, VGLL2_HUMAN, VITO1.

Background: Vgl-2, also known as VITO-1, is a 317 amino acid protein that contains a domain through which it interacts with TEF-1, a protein that plays a role in controlling the expression of numerous genes. Specific to skeletal muscle, Vgl-2 is expressed highly in adult fast muscle and is expressed at lower levels in adult slow muscle and fetal skeletal muscle. During muscle differentiation, Vgl-2 mRNA levels increase and Vgl-2 translocates from the cytoplasm to the nucleus. Overexpression of Vgl-2 in MYOD-transfected 10T1/2 mouse embryonic fibroblasts increases expression of myosin heavy chain (MHC), which is a marker of terminal muscle differentiation. This evidence suggests that Vgl-2 is essential for muscle gene expression. There are two isoforms of Vgl-2 that are produced as a result of alternative splicing events.

Gene ID: 245806