SH2D1A antibody (HRP)

Catalog No. ABIN1423036

Product Details anti-SH2D1A Antibody (HRP)

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH2D1A antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SH2D1A/SAP

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for SH2D1A

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Alternative Name: SH2D1A (SH2D1A Products)

Synonyms: DSHP antibody, EBVS antibody, IMD5 antibody, LYP antibody, MTCP1 antibody, SAP antibody, SAP/SH2D1A antibody, XLP antibody, XLPD antibody, RGD1562408 antibody, Gm686 antibody, SH2D1A antibody, SH2 domain containing 1A antibody, SH2D1A antibody, Sh2d1a antibody

Background:

Synonyms: SH2 domain protein 1A, DSHP, Duncan disease SH2 protein, SAP, Signaling lymphocyte activation molecule associated protein, SLAM associated protein, T cell signal transduction molecule SAP, SH21A_HUMAN.

Background: SH2D1A, also SH2 domain protein 1A, SAP and CD150/SLAM (signaling lymphocyte activation molecule)-associated protein, influences signaling pathways involving SLAM molecules at the interface between T and B cells. SH2D1A modulates SLAM by blocking the recruitment of tyrosine phosphatase SHP2 to the phosphorylated cytoplasmic domain of SLAM. SLAM activation mediates expansion of activated T cells during immune responses, induces production of interferon-?and changes the functional profile of subsets of T cells. SH2D1A is a hydrophilic, 128 amino acid protein that is 96 % homologous to the mouse protein in both SH2 and tail domains. SH2D1A is present in all major subsets of T cells, including CD4+, CD45RO+, CD45RA+ and CD8+, but not in B cells. SH2D1A can interact via an SH2 domain with a motif (TIYXXV) present in the cytoplasmic tail of cell-surface receptors SLAM (CD150), CD84, CD229 (LY9) and CD244 (2B4).

Gene ID: 4068

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process