MUC2 antibody

Catalog No. ABIN1449424

Product Details anti-MUC2 Antibody

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody stains normal colon and colonic carcinomas where it produces intense perinuclear staining in goblet cells. It also reacts with normal and neoplastic breast tissues and with prostate carcinoma. Cellular localization: Cytoplasmic, perinuclear staining of goblet cells.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Protein A Chromatography.

Immunogen: Synthetic MUC2 (23 aa) tandem repeat consensus sequence.

Isotype: IgG

Application Details

Application Notes: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/200 using streptavidin-Biotin system orpolymer system. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM Citratebuffer for 10 min) enhances the signal and may require further dilution of antibody. Positive Control: Human colon carcinoma, LS 174 T colon cancer cell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.0 mg/mL

Buffer: 10 mM PO4, 200 mM NaCl, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for MUC2

Target: MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

Alternative Name: Mucin-2 (MUC2 Products)

Synonyms: 2010015E03Rik antibody, MCM antibody, wnn antibody, MLP antibody, MUC-2 antibody, SMUC antibody, HH-Muc antibody, mucin 2 antibody, mucin 2, oligomeric mucus/gel-forming antibody, mucin-2 antibody, Muc2 antibody, MUC2 antibody, LOC100724045 antibody

Background: Mucin 2, oligomeric mucus/gel-forming, also known as MUC2, is a human intestinal mucin gene. This gene is expressed in colon, small intestine, colonic tumors, bronchus, cervix, gall bladder, and possibly other tissues. Mucins are high molecular weight glycoproteins having oligosaccharides attached to serine or threonine residue of the apomucin protein backbone by O-glycosidic linkages.Synonyms: Intestinal mucin-2, MUC-2, MUC2, SMUC

Gene ID: 4583

NCBI Accession: NP_002448

UniProt: Q02817