POU2AF1 antibody (AA 241-254)

Catalog No. ABIN1491839

Product Details anti-POU2AF1 Antibody

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Binding Specificity: AA 241-254

Reactivity: Human, Horse, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human POU2AF1 / BOB1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin, Bat, Horse (100%) Gibbon, Panda, Dog, Bovine (93%) Elephant, Rabbit (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-DSDAYALNHTLSVE from the C-terminus of human POU2AF1 / BOB1 (NP_006226.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin, Bat, Horse (100%), Gibbon, Panda, Dog, Bovine (93%), Elephant, Rabbit (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000), WB (1 - 2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1 / BOB1 (POU2AF1 Products)

Synonyms: BOB1 antibody, OBF-1 antibody, OBF1 antibody, OCAB antibody, BOB-1 antibody, BOB.1 antibody, Bob-1 antibody, Bob1 antibody, OBF.1 antibody, OCA-B antibody, POU class 2 associating factor 1 antibody, POU domain, class 2, associating factor 1 antibody, POU2AF1 antibody, Pou2af1 antibody

Background: Name/Gene ID: POU2AF1

Synonyms: POU2AF1, BOB-1, OBF-1, OCT-binding factor 1, BOB1, OBF1, OCA-B, OCAB

Gene ID: 5450

NCBI Accession: NP_006226

UniProt: Q16633