Syndecan 1 antibody (Internal Region)

Catalog No. ABIN1493911

Product Details anti-Syndecan 1 Antibody

Target: Syndecan 1 (SDC1)

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Syndecan 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Reacts with the human Syndecan-1 protein. On Western blots, a single band at ~90kD representing the glycosylated form of Syndecan-1, occasionally a band of ~31kD representing the unglycosylated form of Syndecan-1 may be observed. Reactivity has been confirmed with human T47D, SW-480 and PC-3 cell lysates by Western blotting and with formalin-fixed, paraffin-embedded (FFPE) human gastric cancer and breast cancer tissues, and mouse and rat stomach, small bowel, and skin tissues by immunohistochemistry.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from an internal region of the human Syndecan-1 protein.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL, Confirmed with human T47D, SW-480 and PC-3 cell lysates. A single band at ~90kD representing the glycosylated form of Syndecan-1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan-1 may be observed. Immunohistochemistry (FFPE): 10 μg/mL, For best results heat induced epitope retrieval with EDTA,  pH 8.0, is required prior to staining. Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for Syndecan 1

Target: Syndecan 1 (SDC1)

Alternative Name: SDC1 / Syndecan 1 / CD138 (SDC1 Products)

Synonyms: CG10497 antibody, CT27296 antibody, Dmel\\CG10497 antibody, Dsyn antibody, Syd antibody, Syn antibody, dSdc antibody, l(2)10608 antibody, sdc antibody, syd antibody, syn antibody, Syn1 antibody, cd138 antibody, synd1 antibody, xSyn1 antibody, syndecan1 antibody, Syndecan-1 antibody, XSyndecan-1 antibody, SDC1 antibody, CD138 antibody, SDC antibody, SYND1 antibody, syndecan antibody, AA408134 antibody, AA409076 antibody, Sstn antibody, Synd antibody, Synd1 antibody, syn-1 antibody, HSPG antibody, SYNDECA antibody, Syndecan antibody, Syndecan antibody, syndecan 1 antibody, Sdc antibody, sdc1 antibody, SDC1 antibody, Sdc1 antibody

Background: Name/Gene ID: SDC1

Synonyms: SDC1, CD138 antigen, SDC, SYND1, Syndecan, Syndecan 1, Syndecan-1, CD138, Syndecan proteoglycan 1

Gene ID: 6382

UniProt: P18827

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism