FGF1 antibody
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- Target See all FGF1 Antibodies
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FGF1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)
- Brand
- IHC-plus™
- Specificity
- Human FGF-acidic
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human FGF-acidic.
Type of Immunogen: Cell extract - Top Product
- Discover our top product FGF1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (1 - 2.5 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: LS-B8222 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-acidic (10 ng/mL), a concentration of 1-2.5 μg/mL of this antibody is required. ELISA: To detect Human FGF-acidic by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-acidic (LS-C104591) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-acidic. Western Blot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))
- Alternative Name
- FGF1 (FGF1 Products)
- Synonyms
- AFGF antibody, ECGF antibody, ECGF-beta antibody, ECGFA antibody, ECGFB antibody, FGF-1 antibody, FGF-alpha antibody, FGFA antibody, GLIO703 antibody, HBGF-1 antibody, HBGF1 antibody, Fgf1 antibody, FGF1 antibody, aFGF antibody, ecgf antibody, fgfa antibody, ecgfa antibody, ecgfb antibody, fgf-1 antibody, hbgf1 antibody, glio703 antibody, ecgf-beta antibody, fgf-alpha antibody, Dffrx antibody, Fam antibody, Fgf-1 antibody, Fgfa antibody, zgc:136885 antibody, LOC100221393 antibody, fgf1 antibody, zgc:73249 antibody, fibroblast growth factor 1 antibody, fibroblast growth factor 1 L homeolog antibody, fibroblast growth factor 1b antibody, fibroblast growth factor 1a antibody, FGF1 antibody, fgf1.L antibody, fgf1 antibody, Fgf1 antibody, fgf1b antibody, LOC100221393 antibody, fgf1a antibody
- Background
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Name/Gene ID: FGF1
Family: HBGF
Synonyms: FGF1, AFGF, Endothelial cell growth factor, Fibroblast growth factor 1, FGFA, ECGFB, HBGF-1, GLIO703, ECGF, ECGF-beta, ECGFA, FGF-1, FGF-alpha, HBGF1 - Gene ID
- 2246
- UniProt
- P05230
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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