IGFBP5 antibody

Catalog No. ABIN1498816

Product Details anti-IGFBP5 Antibody

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGFBP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human IGF-BP5

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IGF-BP5.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B8207 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. ELISA: To detect hIGF-BP5 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IGF-BP5 (LS-C104305) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIGF-BP5. Western Blot: To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IGFBP5

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGFBP5 / IGFBP-5 (IGFBP5 Products)

Synonyms: IBP5 antibody, AI256729 antibody, AW208790 antibody, IGFBP-5 antibody, IGFBP-5P antibody, IGF-BP5 antibody, igfbp5 antibody, ibp5 antibody, IBP-5 antibody, xIGFBP-5 antibody, IGFBP5 antibody, igfbp5b antibody, zgc:165472 antibody, insulin like growth factor binding protein 5 antibody, insulin-like growth factor binding protein 5 antibody, insulin like growth factor binding protein 5 L homeolog antibody, insulin-like growth factor binding protein 5b antibody, insulin-like growth factor-binding protein 5 antibody, insulin-like growth factor binding protein 5a antibody, IGFBP5 antibody, Igfbp5 antibody, igfbp5.L antibody, igfbp5b antibody, igfbp5 antibody, LOC100194500 antibody, igfbp5a antibody

Background: Name/Gene ID: IGFBP5

Synonyms: IGFBP5, IBP-5, IGFBP-5, IBP5, IGF-binding protein 5

Gene ID: 3488

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding