Insulin Receptor antibody (AA 816-988)

Catalog No. ABIN1498892

The Rabbit Polyclonal anti-Insulin Receptor antibody is suitable to detect Insulin Receptor in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for Insulin Receptor antibody (AA 816-988) (ABIN1498892)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Insulin Receptor Antibody

Binding Specificity: AA 816-988

Specificity: Human Insulin Receptor

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse, Rat (97%).

Purification: Immunoaffinity purified

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 816 and 988 (P06213) of Insulin Receptor. Percent identity by BLAST analysis: Human (100%), Mouse, Rat (97%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR / Insulin Receptor

Background: Name/Gene ID: INSR
Subfamily: Insulin receptor
Family: Protein Kinase

Synonyms: INSR, CD220, ILP receptor, Insulin receptor, IR, HHF5, CD220 antigen

Gene ID: 3643

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity