GCKR antibody (AA 1-214)

Catalog No. ABIN1501890

This anti-GCKR antibody is a Rabbit Polyclonal antibody detecting GCKR in WB, IHC and IHC (p). Suitable for Human and Mouse.

Quick Overview for GCKR antibody (AA 1-214) (ABIN1501890)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GCKR Antibody

Binding Specificity: AA 1-214

Specificity: Human, mouse GCKR

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Rat (92%).

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 214 of GCKR (SwissProt Q14397). Percent identity by BLAST analysis: Human (100%), Rat (92%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, IHC-Paraffin: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GCKR

Background: Name/Gene ID: GCKR

Synonyms: GCKR, GKRP, FGQTL5, Glucokinase regulator, Glucokinase regulatory protein

Gene ID: 2646

UniProt: Q14397

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus