SHP1 antibody (pTyr536)

Catalog No. ABIN1531195

The Rabbit Polyclonal anti-SHP1 antibody is suitable to detect SHP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for SHP1 antibody (pTyr536) (ABIN1531195)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SHP1 Antibody

Binding Specificity: AA 502-551, pTyr536

Specificity:

SHP-1 (Phospho-Tyr536) Antibody detects endogenous levels of SHP-1 only when phosphorylated at Tyr536.

PhosphorylationH:Y536 M:Y536 R:Y538

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human SHP-1 around the phosphorylation site of Tyr536.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.63489 (NCBI Gene Symbol: PTPN6)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for SHP1

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: SHP-1

Background:

Synonyms: 70Z-SHP, HCP, HCPH, Hematopoietic cell protein-tyrosine phosphatase, PTN6, PTP-1C, PTP1C, PTPN6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, Protein-tyrosine phosphatase, non-receptor type 6, SH-PTP1

NCBI Gene Symbol: PTPN6

Molecular Weight: 67 kDa

Gene ID: 5777

OMIM: 176883

UniProt: P29350

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling