FAK antibody (pTyr576)

Catalog No. ABIN1531272

This Rabbit Polyclonal antibody specifically detects FAK in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for FAK antibody (pTyr576) (ABIN1531272)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-FAK Antibody

Binding Specificity: AA 542-591, pTyr576

Specificity:

FAK (Phospho-Tyr576) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr576.

PhosphorylationH:Y576 M:Y614 R:Y576

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr576.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.395482 (NCBI Gene Symbol: PTK2)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: FAK

Background:

Synonyms: FADK 1, FAK1, Focal adhesion kinase 1, pp125FAK, Protein- tyrosine kinase 2, PTK2

NCBI Gene Symbol: PTK2

Molecular Weight: 119 kDa

Gene ID: 5747

OMIM: 600758

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling