Estrogen Receptor alpha antibody (pTyr537)

Catalog No. ABIN1531313

This anti-Estrogen Receptor alpha antibody is a Rabbit Polyclonal antibody detecting Estrogen Receptor alpha in WB, IHC and ELISA. Suitable for Human and Mouse.

Quick Overview for Estrogen Receptor alpha antibody (pTyr537) (ABIN1531313)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 501-550, pTyr537

Specificity:

Estrogen Receptor-alpha (Phospho-Tyr537) Antibody detects endogenous levels of Estrogen Receptor-alpha only when phosphorylated at Tyr537.

PhosphorylationH:Y537 M:Y541 M:Y542

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-alpha around the phosphorylation site of Tyr537.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

Comment:

Unigene-Number: Hs.208124 (NCBI Gene Symbol: ESR1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor-alpha

Background:

Synonyms: ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1

NCBI Gene Symbol: ESR1

Molecular Weight: 66 kDa

Gene ID: 2099

OMIM: 133430

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly