FOXO1 antibody (pSer329)

Catalog No. ABIN1531321

The Rabbit Polyclonal anti-FOXO1 antibody is suitable to detect FOXO1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for FOXO1 antibody (pSer329) (ABIN1531321)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-FOXO1 Antibody

Binding Specificity: AA 295-344, pSer329

Purpose: FoxO1A (phospho Ser329) Antibody

Specificity: Phospho-FoxO1A (S329) Polyclonal Antibody detects endogenous levels of FoxO1A protein only when phosphorylated at S329.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A around the phosphorylation site of Ser329. AA range:295-344

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1A

Background:

Synonyms: FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],

Gene Name: FOXO1

Protein Name: Forkhead box protein O1

Molecular Weight: 69 kDa

Gene ID: 2308

OMIM: 136533

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling