LCK antibody (pTyr505)

Catalog No. ABIN481947

The Rabbit Polyclonal anti-LCK antibody (ABIN481947) specifically detects LCK in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for LCK antibody (pTyr505) (ABIN481947)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-LCK Antibody

Binding Specificity: AA 460-509, pTyr505

Specificity:

Lck (Phospho-Tyr505) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr505.

PhosphorylationH:Y505 M:Y505 R:Y505

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Lck around the phosphorylation site of Tyr505.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:60000

Comment:

Unigene-Number: Hs.470627 (NCBI Gene Symbol: LCK)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: Lck

Background:

Synonyms: kinase Lck, LCK, LSK, LSK-T, lymphocyte-specific protein tyrosine kinase, P56-LCK, T cell-specific protein-tyrosine kinase

NCBI Gene Symbol: LCK

Molecular Weight: 58 kDa

Gene ID: 3932

OMIM: 153390

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling