BAX antibody (pThr167)

Catalog No. ABIN1531490

There are 5+ publications for this product available. The Rabbit Polyclonal anti-BAX antibody is suitable to detect BAX in samples from Human, Mouse and Rat. It has been validated for ELISA and IF.

Quick Overview for BAX antibody (pThr167) (ABIN1531490)

Target: BAX (BCL2-Associated X Protein (BAX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-BAX Antibody

Binding Specificity: AA 133-182, pThr167

Purpose: Bax (phospho Thr167) Antibody

Specificity: Phospho-Bax (T167) Polyclonal Antibody detects endogenous levels of Bax protein only when phosphorylated at T167.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Thr167. AA range:133-182

Isotype: IgG

Application Details

Application Notes: IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

References for anti-BAX antibody (ABIN1531490)

Grossini, Marotta, Farruggio, Sigaudo, Qoqaiche, Raina, de Giuli, Mary, Vacca, Pollastro: "Effects of Artemetin on Nitric Oxide Release and Protection against Peroxidative Injuries in Porcine Coronary Artery Endothelial Cells." in: Phytotherapy research : PTR, (2015) (PubMed).

Grossini, Bellofatto, Farruggio, Sigaudo, Marotta, Raina, De Giuli, Mary, Pollesello, Minisini, Pirisi, Vacca: "Levosimendan inhibits peroxidation in hepatocytes by modulating apoptosis/autophagy interplay." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124742, (2015) (PubMed).

Grossini, Gramaglia, Farruggio, Bellofatto, Anchisi, Mary, Vacca, Zeppegno: "Asenapine increases nitric oxide release and protects porcine coronary artery endothelial cells against peroxidation." in: Vascular pharmacology, Vol. 60, Issue 3, pp. 127-41, (2014) (PubMed).

Grossini, Pollesello, Bellofatto, Sigaudo, Farruggio, Origlia, Mombello, Mary, Valente, Vacca: "Protective effects elicited by levosimendan against liver ischemia/reperfusion injury in anesthetized rats." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 20, Issue 3, pp. 361-75, (2014) (PubMed).

Berger, Quast, Plötz, Kammermeier, Eberle: "Sensitization of melanoma cells for TRAIL-induced apoptosis by BMS-345541 correlates with altered phosphorylation and activation of Bax." in: Cell death & disease, Vol. 4, pp. e477, (2013) (PubMed).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax

Background:

Synonyms: BAX, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

Background: The protein encoded by BAX (BCL2 associated X, apoptosis regulator) belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX.

Gene Name: BAX

Protein Name: Apoptosis regulator BAX

Molecular Weight: 21 kDa

Gene ID: 581

OMIM: 600040

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response